Application

Research CategorySignalingApoptosis & Cancer

Research Sub CategoryGeneral Post-translation Modification

Immunocytochemistry Analysis: 20 µg/mL of this antibody from a representative lot detected Axin2 in HeLa, HEK293 and NIH-3T3 cells.
Western Blotting Analysis: A representative lot of this antibody detected Axin2 in SW 480 cell lines

This Anti-Axin2, clone cond4-1D5 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of Axin2.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Axin-2, or Axis inhibition protein 2, is an inhibitor of the Wnt signaling pathway that down-regulates beta-catenin and APC through phosphorylation. Axin-2 abnormalities are linked to defective mismatch repair and have been found to be involved in colorectal cancer (CRC) and oligodontia-colorectal cancer syndrome (ODCRCS). Axin-2 can be found in brain and lymphoblast tissues.

Immunogen

Linear peptide corresponding to Human Axin 2.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Axin2 in 10 µg of HCT116 cell lysate.

Specificity

This monoclonal antibody recognizes both endogeneous and overexpressed Axin2 and does not cross-react with Axin1

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 95 kDa observed